As the demand for autogenous connective tissue grafts (CTGs) continues to grow, this clinical report introduces a “donor-friendly” and “graft-efficient” soft tissue harvesting technique. Designed to accommodate a broad range of clinicians, this approach streamlines and simplifies the retrieval of connective tissue grafts from the maxillary tuberosity region. A step-by-step illustrated guide provides surgeons a practical roadmap for the seamless execution of the HOT technique (short for ‘Harvesting Oblique Tuberosity’), which is further divided into HOT 1 (regular shape) and HOT 2 (extended shape), depending on the required graft length. By breaking the overall procedure into clearly defined steps, the technique enhances both the predictability and reproducibility of graft outcomes. Additionally, this case series underscores the clinical versatility of the tuberosity replacement graft. Due to the site’s typically volume-rich and collagen-dense characteristics, it supports a wide range of applications in soft tissue phenotype modification therapies.