Objectives: The aim of this study was to evaluate microtensile bond strength of five different self-conditioning dentin adhesives (Futurabond NR, Adper Prompt L-Pop, Contax, AdheSE, Clearfil Protect Bond) on perfused dentin in vitro. Methods: Fifty freshly extracted molars, stored in saline for a maximum of fourteen days after extraction were included. All teeth were specially prepared allowing the simulation of dentin perfusion. Dentin specimens with a total thickness of 3.5 mm were obtained under standardized conditions. The specimens were randomly assigned to five experimental groups of ten samples each: Group A: Futurabond NR; group B: Adper Prompt L-Pop; group C: Contax; group D: AdheSE; group E: Clearfil Protect Bond. Microtensile bond strength was measured 15 minutes after application and light curing of the composite material (Clearfil AP-X, colour A2) using an universal testing machine. Results: For the five test series following bond strengths were evaluated (mean value and standard deviation in MPa): group A: 13.34 (± 4.27), group B: 22.33 (± 2.77), group C: 20.50 (± 3.30), group D: 17.65 (± 4.84), group E: 22.53 (± 3.68). Statistical analysis showed a significant influence of the used material combination on tensile bond strength (pConclusions: Within the limitations of an in vitro investigation it can be concluded that all self-conditionng bonding agents used in this study were able to establish bond strength on perfused dentin showing almost favourable values. Keywords: self-conditioning bonding agent, self-conditioning dentin adhesives, microtensile bond strength