Purpose: To compare the biomechanical effect of splinted versus unsplinted mandibular implants supporting overdentures subjected to experimental static immediate load on bone tissue deformation using strain gauge analysis. Materials and Methods: Strain gauges were bonded on the labial cortical bone adjacent to 2 Straumann dental implants placed in the mandibular interforaminal region of 4 completely edentulous mandibles of fresh human cadavers. The installation torque value (ITV) of each implant was measured using a custom-made torque wrench, and implant stability quotients (ISQs) were also obtained using resonance frequency analysis. Three overdentures (ODs), 2 splinted (bar- and cantilevered bar-retained) and 1 unsplinted (ball-retained), were fabricated for each edentulous mandible. Two experimental loads were applied subsequently via 2 miniature load cells that were placed bilaterally 10 mm (anterior loading) and 15 mm (posterior loading) from the implant. Strain measurements were performed at a sample rate of 10 KHz and under a maximum experimental static load of 100 N; they were simultaneously monitored from a computer connected to a data acquisition system. Finally, the removal torque values (RTV) of the implants were measured. Results: Strains on the labial cortical bone around implants supporting mandibular ODs under anterior loading were significantly higher than measured under posterior loading for all attachment types (P .05). All strain values were compressive in nature, and the minimum strain (-19 µe) was recorded for bar-retained ODs under 25 N posterior loading, while the maximum strain (-797 µe) was for recorded for retentive anchor-retained ODs under 100 N anterior loading. Nonparametric correlations between ISQs, ITVs, and RTVs identified significant correlations only for ITVs and RTVs (P .05). Conclusion: Splinting of 2 interforaminal dental implants, regardless of attachment type, to support mandibular ODs subjected to immediate load significantly reduced initial bone tissue strains experienced on the labial cortical bone in comparison with the use of unsplinted implants. Keywords: biomechanics, dental implants, immediate loading, mandibular overdentures, micromovement, resonance frequency analysis, strain gauges