Purpose: To investigate the physical and mechanical behaviors of polyether ether ketone (PEEK) before and after thermocycling, as well as its potential use as a more durable prosthetic component for implant-supported and -retained removable dental prostheses (I-RDPs). Materials and Methods: Roughness and surface hardness were evaluated in specimens obtained using the subtractive method (n = 20) with a diameter of 9 mm and a thickness of 2 mm, and retention force was measured using attachments with a diameter of 4 mm and a height of 3 mm. For fatigue resistance testing, a polyurethane matrix with two ball abutment implants (MDL, Intra-Lock International) was used to simulate the mandibular alveolar ridge. A total of 40 attachments (n = 20 pairs) were placed in acrylic resin blocks using an analog technique for the direct clinical pickup of overdenture female attachments, then submitted to 2,900 insertion/removal cycles to simulate 24 months of overdenture use. Physical analyses were performed by Fourier-transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), and differential scanning calorimetry (DSC) before and after thermocycling (5°C to 55°C for 10,000 cycles). After normal distribution was verified by Shapiro-Wilk test, one-way ANOVA was applied to analyze the surface roughness and hardness, and two-way ANOVA with Bonferroni adjustment was used to assess the retention force (α = .05). Results: Thermocycling did not change the PEEK surface roughness or hardness (P > .05). As for the retention force, the highest average was observed after the thermocycling test (P = .006). Conclusion: Based on the FTIR, XRD, and DSC results, PEEK crystallinity decreased after thermocycling, and the physical and mechanical behaviors of this polymer were compatible with the proposed application, suggesting that PEEK is a component of greater durability for I-RDPs. Int J Prosthodont 2023;36:612–619.