Objectives: The aim of the present study was to evaluate regional microtensile bond strengths (mTBS) to root canal dentin using different adhesive systems- two dentin adhesives, one resin cement and one adhesive root canal sealer. Methods: 120 human single rooted teeth were used. Crowns were removed at the CEJ. The roots were bisected along their long axis and sectioned into three thirds: apical (a), middle (m) and coronal (c). Flat root dentin specimens were prepared. The specimens were randomly assigned to four experimental groups of ten samples each: Group S: Syntac; group F: Futurabond NR; group P: Panavia F 2.0; group R: Resilon. The different adhesive materials were applied as recommended by the manufacturer. A composite build up (Ø 1mm) was created over each root canal section to allow mTBS tests. Bond strength was measured using a special device in an universal testing machine. Results: Statistical analysis showed a significant influence of the used materials and the different regions on mTBS (p Keywords: bond strength, root canal dentin, adhesives