The purpose of this investigation was to develop a new, in vitro bacteria-yeast denture colonization model. Candida albicans and Streptococcus mutans, organisms associated with denture stomatitis, reproducibly colonized denture blanks in high numbers. Both Efferdent and Super-Strength Polident were able to substantially reduce or eliminate colonizing S mutans. In sharp contrast, however, no significant decreases in adherent C albicans were observed with either denture soaking agent.