Chemical cleansing has been suggested as one of the most effective methods for plaque control, particularly ffor resilient denture-lining materials. These materials are known to be easily colonized and infected by Candida albicans. Maintaining the cleanliness of such materials is difficult because of porosity and incompatibility with some types of denture cleasers. Therefore, denture cleansers used for soft liners must address both microbiologic and physical requirements. This study evaluated the antifungal efficacy of 11 commercial denture cleansers on C albicans biofilm, studied the compatibility of tissue conditioners with denture cleansers, and makes suggestions for the chemical cleansing of dentures lined with tissue conditioners.