Purpose: Removable partial dentures (RPDs) are widely used in clinical dental practice as a cost-effective treatment to restore the oral function. Clear understanding of the effect of partial dentures on masticatory performance is important for clinical decision making in prosthodontics. The purpose of this systematic review is to evaluate the impact of RPDs on masticatory performance (MP) of partially edentulous subjects. Material and methods: Electronic database and hand searches were conducted to explore clinical trials reporting the evidence of the effect of metal and acrylic based removable partial dentures on mastication. The validated objective and subjective measures of assessment of mastication were included. The article selection, data extraction and assessment of risk of bias were conducted by two reviewers independently. Results: Out of 11200 studies, one randomised control trial and 23 clinical trials met the inclusion criteria. Positive impact on different parameters of masticatory function after RPD treatment, was reported in 14 studies. Subjective evaluation of chewing ability (CA) displayed an improvement in 83.3% of studies, while improvement was evidenced only in 69.5% of studies upon objective evaluation. Majority of the studies were reporting data on free end saddles. Out of the 13 studies associated with bilateral free end saddle dentures, 9 studies showed a positive effect on masticatory efficiency. Various factors were found to have an impact on the masticatory performance (MP) of RPDs. Conclusion Subjective evaluation displays higher positive impact of RPDs in MP than objective evaluation. Assessment of patients’ perspectives is important in clinical decision making. Multiple factors affect the MP with RPD treatment. I