Objectives: The aim of this study was to investigate the efficacy of a novel desensitizing paste based on Pro-Argin™ technology (GABA GmbH) on hypersensitive teeth before and after professional tooth cleaning. Materials and Methods: A total of 26 male and female patients (aged between 23 and 67 years, middle 45 years) with established dentin hypersensitivity (as least one hypersensitive tooth with a tactile sensitivity score) were randomly assigned in two groups. Group 1: before PTC the paste was applied; group 2: after PTC the paste was applied as final polishing step. Before starting the procedure, the tooth was stimulated 1 sec. with air blast and then the patient was asked to report the sensitivity (rating scale: slight, partial, moderate, severe). The desensitizing paste was applied to the gingivo-facial third of the selected tooth (or teeth) using a rotating prophy cup. The tested tooth (teeth) was (were) re-stimulated as prior to treatment and the patient was asked to describe any change in sensitivity (rating scale: total, improved, none). Results: After treatment with the desensitizing paste 42 of 50 teeth yielded reduction in dentin hypersensitivity. Group 1 showed an improvement in 20 of 28 teeth (71, 5%). Thereof 11 teeth were completely improved and 9 teeth conceived advancement. 8 teeth (28,5 %) showed no improvement of dentin hypersensitivity. Group 2 showed 16 completely and 6 partially improved teeth. Conclusions: Within the limitation of this first clinical survey and in consideration of the individual sensation of pain, it can be concluded that this desensitizing paste achieved good successes on hypersensitivity cervical areas when applied before and after PTC. However the effectiveness could be influenced through mechanical manipulation. Keywords: dentin hypersensitivity, dentin, pain, desensitizing paste, clinical survey