Aim: It was the purpose of the study to examine the effect of two desensitizers (Hyposen, lege artis Pharma GmbH, and the upon Pro-Argin ™ technology based Elmex® Sensitive Professional™ Desensitizing Paste, GABA) on the microtensile strength of an adhesive system (Bifix QM, Voco) after artificial aging by water storage and application of a temporary cement (Provicol QM, Voco). Material and method: The preparation of 105 dentin specimens permitted the simulation of the dentin perfusion and application of the materials under standardised conditions. The specimens were divided by chance into seven experimental groups of 15 each: 1: Control without desensitizers; 2: Hyposen; 3: Hyposen, water storage; 4: Hyposen, Provicol, water storage; 5: Paste application; 6: Paste application, water storage; 7: Paste application, Provicol, water storage. The groups 3, 4, 6 and 7 were stored one week in saline. In the groups 4 and 7 the temporary cement was applied additionally after the application of the desensitizers. The microtensile bond strength was determined using an universal test machine. Results: For the test rows the following values were determined (averages and standard deviation in MPa): 1: 14.04 (±2.64), 2: 13.16 (±2.12), 3: 13.68 (±2.77), 4: 12.27 (±2.42), 5: 12.20 (±1.94), 6: 12.68 (±1.72), 7: 10.61 (±1.85). The statistical analysis showed a significant influence of the used desensitizers and the aging procedures on microtensile bond strength (p 0.001, ANOVA). In comparison to the control group the application of both desensitizers and water storage led not to a significant reduction of the tensile bond strength. Compared to the control group additional application of the temporary cement reduced the tensile strength in the group 7 significantly (p 0.05, Tukey\'s test). Conclusion: Within the limitations of an in vitro investigation it cn be concluded, that the application of both Desensitizer does not influence the tensile bond strength of the used adhesive system. Indeed, the additional use of a temporary cement can influence tensile bond strength. Keywords: dentin bonding agents, dentin, bond strength, desensitizer, dentin hypersensitivity