Objectives: The aim of the present study was to evaluate the influence of a densensitizer (Gluma Desensitizer) on tensile bond strength of different dentin adhesives. Methods: Sixty freshly extracted third molars stored in saline for a maximum of seven days after extraction were included in this study. All teeth were specially prepared allowing the simulation of dentin perfusion. Dentin specimens with a total thickness of 3.5 mm were obtained under standardized conditions. The specimens were randomly assigned to four experimental groups: group A1: Clearfil New Bond/ Clearfil Core; group A2: Gluma Desensitizer/ Clearfil New Bond/ Clearfil Core; group B1: Xeno III/ Tetric; group B2: Gluma Desensitizer/ Xeno III/ Tetric. All materials were applied as recommended by the manufacturer. Tensile bond strength of the above mentioned bonding agents was measured 15 minutes after application and light curing of the composite material (colour A2) using an universal testing machine. Results: For the four test series following tensile bond strengths were evaluated (mean values and standard deviations in MPa): Group A1: 12.59 (± 4.65), group A2: 12.50 (± 5.26), group B1: 4.46 (± 1.38), group B2: 6.60 (± 1.23). Statistical analysis showed a significant influence of the used dentin bonding agent on tensile bond strength (pConclusions: Within the limitations of an in vitro investigation it can be concluded that the pretreatment of dentin using a desensitizer might not affect tensile bond strength of the dentin adhesives tested. Keywords: bond strength, dentin, dentin adhesive, desensitizing