This study aimed to evaluate the perception of lip position preferences in a Saudi population and compare it to the perception of other populations. Master pictures of a male and female Saudi model were prepared with the upper lip 3 mm from Ricketts' E-line and the lower lip 1 mm behind. The lip position was then altered using computer imaging to create a range of pictures from the upper lip 15 mm behind the E-line to the upper lip 6 mm beyond the E-line. One hundred and fifty-six individuals (60 male, 96 female) were asked to rate the pictures in terms of acceptability and also which picture they liked best. The preferences were compared based on gender, perception of their own lateral profile and history of previous orthodontic treatment. The study found that regardless of gender, the Saudi population has a distinct preference for lip positions that are further away from the E-line than the current Western standard. The population studied also showed a greater tolerance for deviation in the lip position away from the E-line than deviations towards the E-line.