Pages 31-37, Language: EnglishManiatopoulos, C.An improved method for preparing ground tissue-implant sections of good quality for light microscopy is presented. The specimen is embedded in methacrylate and cut using a diamond wafering saw. The cut surface is polished and stained with Stevenel's blue and a glass microscope slide is glued to the surface of the specimen using epoxy resin adhesive. A section is prepared, reduced to 30 to 50 µm using petrographic grinding techniques and protected by a glass coverslip. This method provides high quality sections in which fine tissue architecture, cellular detail, and the tissue-implant interface are preserved.
