In vitro Besiedlung verschiedener Matrixmaterialien mit porcinen Chondrozyten

Objectives: The choice of the appropriate scaffold is a major focus in chondral tissue engineering. Materials and Methods: Porcine chondral cells were expanded by cell culture. Five different materials served as scaffolds: TissueFleece, Bio-Oss®, Chondro-Gide®, Hydoxylapatite (HA) and Tricalcium Phosphate (TCP). Cell vitality was assessed by fluorescin diacetate (FDA) and propidium iodide (PI) staining, biocompatibility with LDH and BrdU, MTT, WST tests and scanning electron microscopy (SEM). Results: All probes showed viable chondral cells. All biocompatibility tests revealed that proliferation on all scaffolds was similar to controls. Chondral cells formed layers covering the surfaces of all five scaffolds in SEM. Keywords: tissue engineering, Knorpel, Biokompatibilität, Matrixmaterialien