Purpose: Methods for removing temporary cement with an antibacterial effect might improve prosthetic treatment prognosis. Materials and Methods: Three removal methods were assessed: (1) immersion of an acrylic provisional restoration in a temporary cement dissolver (Temp-off); (2) mechanical removal with a dental explorer; and (3) sandblasting. Two temporary cements, one with eugenol (Temp-Bond) and one without eugenol (Temp-Bond NE) and of two thicknesses (100 μm and 250 μm), were examined. Results: Immersion in Temp-off produced the most effective bacterial count decrease compared to the other methods (P .05). No significant difference was found between the two cement types, although the decrease in count was more evident in the thicker (250 μm) cement layer. Conclusion: Temp-off dissolving liquid used for removal of temporary cement exhibited an antibacterial effect and nonmechanical cleaning ability.