Duplicate complete dentures can be used as custom trays and occlusion rims to fabricate biologically harmonious complete dentures. However, no reports have described the use of duplicated removable partial dentures to achieve simultaneous impressions of the alveolar ridge and teeth, along with bite registration. This case report describes the use of 3D-printed duplicated removable partial dentures with removable split custom trays for denture fabrication. This technique can address the challenges associated with adapting to newly fabricated dentures.