Microscopic and Histochemical Evaluation of Demineralized Freeze-dried bone Allograft in Association with Implant Placement: A Case Report

A case report is presented in which a titanium implant was placed into a defect resulting from the extraction of an impacted tooth; the defect was filled with demineralized freeze-dried bone allograft particles without a membrane barrier. After a 6-month healing period the bone defect had completely healed and the tissue present had macroscopic features similar to mature bone. Histologic examination of this tissue showed that, in all demineralized freeze-dried bone allograft particles, mineralization nodules were scattered inside the demineralized bone; in the areas where the mineralization nodules were present, osteocyte lacunae could be observed. In the case presented, significant new vital bone formation was observed 6 months after placement of a demineralized freeze-dried bone allograft.