Purpose: To determine and compare the frequency of contamination on different sites of

healing abutments (HAs) after sterilization with Phloxine B dye on unused and used HAs after

sterilization. Materials and Methods: A total of 60 HAs were divided into two test groups: (1)

used and sterilized and (2) a control group (unused). The test group was evaluated for

contamination after sterilization; the control group did not undergo any procedure. Data were

analyzed using SPSS version 22 (IBM). Descriptive statistics were used to determine the

frequency of contamination in the different groups and at the different HA sites. Chi-square

test was used to evaluate the association of frequency of contamination with the HA site and

design. The level of significance was kept at P ≤ .01. Results: The test group showed more

contamination compared to the control group. The most contaminated sites were the screw

thread and the screwdriver engagement location. Conclusions: HA reuse is cost-effective, but

cleaning and sterilization was not effective for these components; thus, HAs need to be reused

with caution because they were heavily contaminated when compared to new HAs. Among 

the different sites, the screw thread and screwdriver engagement sites were more prone to

contamination. HA type did not influence the frequency of contamination.