The purpose of this study was to evaluate whether regeneration is possible in human furcation defects. Three molars with Class II furcation involvement were included in this study. A notch was placed in calculus in the furcation to serve as a reference point for histologic evaluation. The defects were treated with a demineralized freeze-dried bone allograft combined with a bioabsorbable membrane. At 6 months postoperative, the teeth were extracted with a small piece of tissue from the furcation area. The samples were then processed for histologic evaluation. Regeneration was found in 2 of the 3 samples. This study demonstrated that regeneration is possible in human furcation defects.