Purpose: To evaluate the roughness and color stability of different types of resins used to immerse denture bases in various denture cleansers overnight. Materials and Methods: A total of 150 resin samples were made, which were divided into three groups of denture resins (conventional thermally activated, milled, and 3D printed; n = 50) and subdivided into five subgroups of denture cleaners (Distilled water, Corega tabs, Efferdent, NaOCl 1%, Listerine Cool Hint; n = 10). The roughness properties (Ra) and chromatic difference (ΔE₀₀) were evaluated during 90 days and 180 days of overnight cleaning. Data were evaluated with two way ANOVA and Tukey tests for multiple comparisons (P < .05). Results: Listerine had significantly higher Ra and ΔE values (P < .001) compared to other solutions, which was significant in conventional resin at 180 days (P < .001). Conclusions: The use of Listerine as an overnight cleaner presents greater damage to the optical and surface properties of denture base resins, mainly with conventional resin, and 1% NaOCl is a valid option in relation to cost benefit.