DOI: 10.3290/j.jad.a29588, PubMed ID (PMID): 23593641Pages 541-546, Language: EnglishRodriguez, Ismael A. / Ferrara, Carlos A. Rozas / Campos-Sanchez, Fernando / Alaminos, Miguel / Echevarría, Jorge Uribe / Campos, AntonioPurpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA).
Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used.
Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures.
Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts.
Keywords: glass-ionomer cements, biocompatibility, intracellular, electron probe microanalysis, human gingival fibroblast, LDH
