The shift from reparative to regenerative dentistry reflects the current trend in medicine and also mirrors the new understanding of caries as a chronic disease. Selfassembling peptide P11-4 showed promising results in the regenerative biomimetic remineralization of initial carious lesions. This approach may present safe and acceptable preventive and minimal invasive treatment for initial caries in children and adolescents. Further studies it is suggested to investigate this novel approach in treatment of molar incisor hypomineralization and cavitated carious lesions. Keywords: P11-4, biomimetic remineralization, caries prevention, enamel, minimal invasive treatment, pediatric dentistry, self-assembling peptide