Purpose: To investigate the aging and fracture resistance of screw-retained implant-supported single crowns (iSCs) fabricated from lithium disilicate containing virgilite (VLD). Materials and Methods: iSCs were fabricated from VLD (CEREC Tessera, Dentsply Sirona) and lithium disilicate (control, n = 8; IPS e.max CAD, Ivoclar) and bonded to a Ti-base abutment. VLD crowns were luted either with (VLDc, n = 8) or without (VLDw, n = 8) a coupling agent. iSCs were cleaned, sterilized, and screw-retained on implants. Fracture force was determined after thermal cycling and mechanical loading (TCML). Results: All crowns survived TCML. No significant differences in mean fracture forces were identified between VLDc (1,583 N), VLDw (1,694 N), and control (1,797 N; P = .639 ANOVA, P = 1.000 Bonferroni). Conclusions: Screw-retained iSCs fabricated from VLD provide acceptable stability, which is independent on the usage of a coupling agent for bonding to Ti-base abutments and sterilization.