PMID- 36259438 OWN - Quintessence Publishing Company, Ltd. CI - Copyright Quintessence Publishing Company, Ltd. OCI - Copyright Quintessence Publishing Company, Ltd. TA - Oral Health Prev Dent JT - Oral Health and Preventive Dentistry IS - 1757-9996 (Electronic) IP - 1 VI - 20 PST - epublish DP - 2022 PG - 355-362 LA - en TI - Effect of Lemon Essential Oil Microemulsion on the Cariogenic Virulence Factor of Streptococcus mutans via the Glycolytic Pathway LID - 10.3290/j.ohpd.b3464891 [doi] FAU - Luo, Shuyu AU - Luo S FAU - Feng, Chong AU - Feng C FAU - Zheng, Yafei AU - Zheng Y FAU - Sun, Yanwe AU - Sun Y FAU - Yan, Changqing AU - Yan C FAU - Zhang, Xiangyu AU - Zhang X CN - OT - dental caries OT - lemon essential oil microemulsion OT - Streptococcus mutans OT - acid production OT - lactate dehydrogenase AB - Purpose: To investigate the effects and mechanisms of lemon essential oil products on dental caries prevention. Materials and Methods: Lemon essential oil microemulsions (LEOM) with concentrations of 1/8 minimum inhibitory concentration (MIC), 1/4 MIC, and 1/2 MIC were applied to S. mutans at concentrations of 0.2%, 1%, and 5% glucose, respectively. Changes in acid production capacity of S. mutans were measured based on changes in pH. The effect of the reductive coenzyme I oxidation method on LDH activity was examined. The effect of lemon essential oil microemulsion on the expression of the lactate dehydrogenase gene (ldh) was detected by a quantitative real-time polymerase chain reaction. Results: Lemon essential oil microemulsion at 1/2 MIC concentration reduced the environmental pH value at different glucose concentrations, compared to those observed in the control group (p  AID - 3464891